With the introduction of fluorogenic probes and fluorometric thermal cyclers that enable the monitoring of amplification reactions in real time, the sensitivity and precision of gene expression ...
Multicenter, randomized, phase III trial comparing radical retropubic prostatectomy with conventional external beam radiotherapy for localized prostate cancer: An interim report ...
Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of ...
The introduction of real-time quantitative PCR (qPCR) in the early 1990s opened the door for quantitative gene expression analysis. Still, qPCR depends on standard curves, allowing only relative ...
Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...
Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...
Data of cDNA microarrays were obtained from 18 pairs of cancerous and noncancerous gastric tissues. Nine patients who survived > 30 months were identified as good survival, and the other nine, who ...
An important and necessary consideration in any quantitative PCR gene expression analysis is the selection of an optimal control gene to correct for differences in RNA sampling. This Infographic ...
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