The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...
TIRF is a microscopy technique that is used to image fluorescent molecules, such as green fluorescent protein (GFP) and fluorochromes, in liquids that are adjacent to a solid with a high refractive ...
In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...
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