SOLVED: Lab 9 PCRPre-Lab Questions:What do you think would happen to primer binding to the template if the annealing temperature is too high? How would PCR be affected if we have primers that have very different annealing temperatures? Why do we add MgCl2 to the reaction mix?Post-Lab Questions:Why is the first denaturation step longer than the other denaturation steps? Why is the binding of the 3' end of the primer to the template more important than the binding of the 5' end?
SOLVED: Lab 9 PCRPre-Lab Questions:What do you think woul…
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